High-intensity focused ultrasound (HIFU) involves the transmission of an ultrasonic beam that is focused within the body to induce tissue necrosis without damaging intervening tissues. HIFU can be used to occlude umbilical artery blood flow in fetal rabbits. HIFU exposure brought about vacuolar degeneration and destruction of elastic fibers in the tunica media of the artery. When HIFU was applied at 5.5 kW/cm 2, blood flow was completely occluded within 15 courses. The mean diameter of the fetal umbilical artery was 0.6 ± 0.2 mm and the mean peak systolic velocity of arterial blood flow was 44.7 ± 18.5 cm/s. Fetal necropsy was performed and exposed lesions were assessed by microscopic histological analysis. Courses of 5-s HIFU exposure were performed until cessation of umbilical blood flow and cardiac arrest were confirmed by Doppler ultrasonography. Using this transducer, HIFU was applied at 1.4, 2.75 or 5.5 kW/cm 2 through the maternal abdominal skin to the fetal intra-abdominal umbilical arteries of four time-mated Japanese White rabbits (11 fetuses) on gestational day 25. MethodsĪ prototype HIFU transducer in combination with an imaging probe with Doppler capability was constructed. ![]() To investigate the application of high-intensity focused ultrasound (HIFU) for fetal umbilical artery blood flow occlusion in a rabbit model.
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